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48-well modified boyden chamber  (Neuro Probe)

 
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    Neuro Probe 48-well modified boyden chamber
    Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of <t>a</t> <t>modified</t> 48-well <t>Boyden</t> chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.
    48 Well Modified Boyden Chamber, supplied by Neuro Probe, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+48-well+boyden+chamber/pmc11903430-97-8-10?v=Neuro+Probe
    Average 90 stars, based on 1 article reviews
    48-well modified boyden chamber - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Multitargeted biological actions of polydatin in preventing pseudogout acute attack"

    Article Title: Multitargeted biological actions of polydatin in preventing pseudogout acute attack

    Journal: Frontiers in Molecular Biosciences

    doi: 10.3389/fmolb.2025.1553912

    Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of a modified 48-well Boyden chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.
    Figure Legend Snippet: Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of a modified 48-well Boyden chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.

    Techniques Used: Migration, Clinical Proteomics, Positive Control, Negative Control, Membrane, Modification



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    Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of a modified 48-well Boyden chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Multitargeted biological actions of polydatin in preventing pseudogout acute attack

    doi: 10.3389/fmolb.2025.1553912

    Figure Lengend Snippet: Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of a modified 48-well Boyden chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.

    Article Snippet: Chemotaxis experiments were performed in a 48-well modified Boyden chamber (Neuro Probe, Gaithersburg, MD) using 5 μm nucleo-pore polyvinyl pyrrolidine-free polycarbonate filters.

    Techniques: Migration, Clinical Proteomics, Positive Control, Negative Control, Membrane, Modification